In vitro axillary shoot regeneration from cotyledonary node of Bishop’s weed (Trachyspermum ammi L.)

Bishop’s seed (Trachyspermum ammi L.) is a vital neutraceutial plant used as spice and also medicinal for curing different ailments diseases of humans animals. The study presents the in vitro regeneration using cotyledonary node explant taken from germinated seeds. All mediums this were comprised MS (0.44%) medium having 3.0%sucrose 0.65% agar with pH 5.8. Expalnts inoculated on enriched 0.10-1.60 mg/L Thidiazuron (TDZ) alone or combination 0.10 IBA (Indole-3-butyric acid) seven weeks under 16/8 light photoperiod provided cool-white fluorescent lamps. Thereafter, explants sucultured without any growth regulators (PGRs) five more similar culture conditions. Results revealed 100% shoot frequency 42.85-100.00%. count length ranged 3.43-19.40 1.04-2.74 cm respectively. highest (19.40) (2.74 cm) observed Ms 0.20 TDZ TDZ+0.10 Relatively low rooting was recorded containing adaptation percentage plantlets pots peat moss.